Urine toxicological screening method using an immunoenzymatic assay based on a biochip array technology: A comparative study of immunochromatography and liquid chromatography–mass spectrometry (notice n° 135246)
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| fixed length control field | 02796cam a2200313 4500500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250112021256.0 |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | fre |
| 042 ## - AUTHENTICATION CODE | |
| Authentication code | dc |
| 100 10 - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Metsu, David |
| Relator term | author |
| 245 00 - TITLE STATEMENT | |
| Title | Urine toxicological screening method using an immunoenzymatic assay based on a biochip array technology: A comparative study of immunochromatography and liquid chromatography–mass spectrometry |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2022.<br/> |
| 500 ## - GENERAL NOTE | |
| General note | 80 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Multiparametric toxicology research is mainly based on immunochromatography (IC) and chromatography methods. A new automated method using an immunoenzymatic (IE) assay based on biochip array technology combines a short turnaround time and analytical performances similar to chromatography in terms of positivity cut-off. The aim of our study was to compare IE with IC and chromatography methods using urine samples from clinical cases. Seventy-two samples were analyzed using IC (amphetamines, opiates, benzodiazepines, THC, methadone, cocaine), IE, and chromatography (previous classes plus opioids and cathinone). The IC results were read by at least 7 operators to assess reading subjectivity. The IE, IC, and chromatography results were compared with each other. Chromatographic quantification was analyzed to understand discrepancies. Significant discrepancies (29–64%) were observed between IC and IE for most of the drug families investigated except for benzodiazepines, methadone, and opiates. These discrepancies were not identified between IE and chromatography, except for some substances (28%–67% discrepancies for buprenorphine, tramadol, and oxycodone, 100% for cathinone). In contrast to IC, the performance of IE neared those of chromatography, except for some substances for which cross-reactions need to be investigated. Reading discrepancies were frequent with IC (33% of samples) and made robust result output challenging. In conclusion, Multistat® is an interesting method for first-line toxicological screening for laboratories that don’t use the chromatography method. |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Nouhaud, Malika |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | El-Balkhi, Souleiman |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lavit, Michel |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Paillard, Hélène |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Berthomès, Pierre |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Martinez, Chantal |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gicquel-Bordelongue, Nathalie |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Blonstein, Benjamin |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Latier, Julien |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Billoré, Sabine |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chassagne, Claire |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lanot, Thomas |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Février, Frédéric |
| Relator term | author |
| 786 0# - DATA SOURCE ENTRY | |
| Note | Annales de Biologie Clinique | 80 | 4 | 2022-07-01 | p. 369-384 | 0003-3898 |
| 856 41 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://shs.cairn.info/journal-annales-de-biologie-clinique-2022-4-page-369?lang=en">https://shs.cairn.info/journal-annales-de-biologie-clinique-2022-4-page-369?lang=en</a> |
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