Comparison of two SARS-CoV-2 RT-PCR assays and the implication of the instrument software on the cycle threshold (Ct) value (notice n° 136091)
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| fixed length control field | 01615cam a2200229 4500500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250112021453.0 |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | fre |
| 042 ## - AUTHENTICATION CODE | |
| Authentication code | dc |
| 100 10 - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Farfour, Eric |
| Relator term | author |
| 245 00 - TITLE STATEMENT | |
| Title | Comparison of two SARS-CoV-2 RT-PCR assays and the implication of the instrument software on the cycle threshold (Ct) value |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2022.<br/> |
| 500 ## - GENERAL NOTE | |
| General note | 47 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The Cycle Threshold (Ct) value of SARS-CoV-2 RT-PCRs is used as an indicator of viral load. Using a collection of 45 fresh nasopharyngeal samples collected on universal transport media, we compare the Ct value obtained with 2 RT-PCR assays, the Alinity M SARS-CoV-2, and the Alinity M RESP-4-Plex (Abbott Molecular, Des Plaines, Illinois, United States) processed on an Alinity M device. The assays are highly correlated; however, the Ct values were a median of 4.54 lower with the Alinity M RESP-4-Plex. This difference could be attributed to earlier detection of positivity by the Alinity M software rather than a difference in RT-PCR performances. The Ct-value of SARS-CoV-2 RT-PCRs should be interpreted with caution, taking into account the clinical context and pre-analytical and analytical findings. |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pascreau, Tiffany |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zia-Chahabi, Sara |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Jolly, Emilie |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ferrière, Patrice |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mazaux, Laurence |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Vasse, Marc |
| Relator term | author |
| 786 0# - DATA SOURCE ENTRY | |
| Note | Annales de Biologie Clinique | 80 | 6 | 2022-12-01 | p. 537-540 | 0003-3898 |
| 856 41 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://shs.cairn.info/journal-annales-de-biologie-clinique-2022-6-page-537?lang=en">https://shs.cairn.info/journal-annales-de-biologie-clinique-2022-6-page-537?lang=en</a> |
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