MMLV reverse transcriptase, a relevant tool for molecular biology (notice n° 1529016)
[ vue normale ]
| 000 -LEADER | |
|---|---|
| fixed length control field | 01438cam a2200181 4500500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20251012013253.0 |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | fre |
| 042 ## - AUTHENTICATION CODE | |
| Authentication code | dc |
| 100 10 - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Lopez, Eva |
| Relator term | author |
| 245 00 - TITLE STATEMENT | |
| Title | MMLV reverse transcriptase, a relevant tool for molecular biology |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2025.<br/> |
| 500 ## - GENERAL NOTE | |
| General note | 78 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The reverse transcriptase of Moloney murine leukemia virus (MMLV) is an enzyme that synthesizes DNA from an RNA template. Among reverse transcriptases, this enzyme is currently the most commonly used in molecular biology and diagnostics. Since its discovery, this viral protein has been extensively studied, shedding light on its structural and functional characteristics, and offering opportunities to optimize its catalytic performance for biotechnological applications. The review describes the structural motifs of the reverse transcriptase of MMLV, the key amino acids in enzymatic catalysis, and the enzyme’s properties such as processivity, fidelity, and thermal stability. This review reports the optimizations made to improve these parameters, which enabled the modified enzymes to be integrated in the molecular biology tests used in the laboratory on a daily basis. |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Valle, Coralie |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Coutard, Bruno |
| Relator term | author |
| 786 0# - DATA SOURCE ENTRY | |
| Note | Virologie | 29 | 1 | 2025-03-12 | p. 7-18 | 1267-8694 |
| 856 41 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://shs.cairn.info/journal-virologie-2025-1-page-7?lang=en&redirect-ssocas=7080">https://shs.cairn.info/journal-virologie-2025-1-page-7?lang=en&redirect-ssocas=7080</a> |
Pas d'exemplaire disponible.
Réseaux sociaux