MALDI-TOF mass spectrometry identification and antifungal susceptibility testing of yeasts causing vulvovaginal candidiasis (VVC) in Tebessa (Northeastern Algeria) (notice n° 1725196)
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| fixed length control field | 04401cam a2200337 4500500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20260322002633.0 |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | fre |
| 042 ## - AUTHENTICATION CODE | |
| Authentication code | dc |
| 100 10 - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Benhadj, Mabrouka |
| Relator term | author |
| 245 00 - TITLE STATEMENT | |
| Title | MALDI-TOF mass spectrometry identification and antifungal susceptibility testing of yeasts causing vulvovaginal candidiasis (VVC) in Tebessa (Northeastern Algeria) |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2023.<br/> |
| 500 ## - GENERAL NOTE | |
| General note | 2 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Vulvovaginal candidiasis (VVC) alongside with antifungal resistance are becoming a major clinical problem in recent years. A prospective study aimed to evaluate the diversity of yeast strains associated with VVC in Tebessa city (northeastern Algeria) and investigate their susceptibility patterns. Over two months, yeasts were isolated on chromogenic medium from twenty-nine non-pregnant women with symptomatic VVC. The isolates were characterized with MALDI-TOF MS and antifungal susceptibility testing was performed for nine antifungal drugs using SensititreTM YeastOneTM YO10. Twenty-nine non-duplicate yeasts were recovered and the mass spectrometry profiles showed reliable scores of which four genera and five different species were identified. Candida albicans accounted for 65.5 % (n = 19) of the total number of isolates, followed by C. glabrata with 20.7% (n = 6). For the remaining non-albicans Candida (NCA) species, Kluyveromyces marxianus with 6.9% (n = 2), Pichia kudriavzevii and Saccharomyces cerevisiae with one isolate each. The antifungal susceptibilities showed wild type MICs of C. albicans to amphotericin B, azoles and echinocandins. In addition, four C. albicans isolates were resistant to flucytosine. For C. glabrata isolates, 100% non-WT phenotype was found for both posaconazole and itraconazole. For the very first time, the obtained outcomes bring out new data concerning the epidemiology of yeasts causing VVC in Algeria and their antimicrobial susceptibility profiles. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | La candidose vulvo-vaginale (CVV) et la résistance aux antifongiques sont devenues un problème clinique majeur ces dernières années. Une étude prospective visait à évaluer la diversité des souches de levure associées à la VVC dans la ville de Tebessa (nord-est de l’Algérie) et à étudier leurs profils de sensibilité. Pendant deux mois, des levures ont été isolées sur un milieu chromogène à partir de vingt-neuf femmes non enceintes présentant une VVC symptomatique. Les isolats ont été caractérisés par MALDI-TOF MS et des tests de sensibilité antifongique ont été réalisés pour neuf médicaments antifongiques à l’aide du SensititreTM YeastOneTM YO10. Vingt-neuf levures non dupliquées ont été récupérées et les profils de spectrométrie de masse ont montré des scores fiables parmi lesquels quatre genres et cinq espèces différentes ont été identifiés. Candida albicans représentait 65,5 % (n = 19) du nombre total d’isolats, suivi de C. glabrata avec 20,7 % (n = 6). Pour les autres espèces de Candida non albicans (NCA), Kluyveromyces marxianus avec 6,9 % (n = 2), Pichia kudriavzevii et Saccharomyces cerevisiae avec un isolat chacun. Les sensibilités antifongiques ont montré des CMI de type sauvage de C. albicans à l’amphotéricine B, aux azoles et aux échinocandines. En outre, quatre isolats de C. albicans étaient résistants à la flucytosine. Pour les isolats de C. glabrata, un phénotype 100% non WT a été trouvé à la fois pour le posaconazole et l’itraconazole. Pour la première fois, les résultats obtenus apportent de nouvelles données concernant l’épidémiologie des levures responsables de la VVC en Algérie et leurs profils de sensibilité aux antimicrobiens. |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Algérie |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | C. albicans |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | C. glabrata |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | candidose vulvovaginale |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | sensibilité aux antifongiques |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | spectrométrie de masse MALDI-TOF |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Algeria |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | antifungal susceptibility |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | C. albicans |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | C. glabrata |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | MALDI-TOF Mass spectrometry |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Vulvovaginal Candidiasis |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Menasria, Taha |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Ranque, Stéphane |
| Relator term | author |
| 786 0# - DATA SOURCE ENTRY | |
| Note | Annales de Biologie Clinique | 81 | 6 | 2023-11-01 | p. 576-584 | 0003-3898 |
| 856 41 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://stm.cairn.info/revue-annales-de-biologie-clinique-2023-6-page-576?lang=en&redirect-ssocas=7080">https://stm.cairn.info/revue-annales-de-biologie-clinique-2023-6-page-576?lang=en&redirect-ssocas=7080</a> |
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