Contribution of a molecular test for the diagnosis of genital infection with Trichomonas vaginalis and Mycoplasma genitalium (notice n° 274500)
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| fixed length control field | 03327cam a2200325 4500500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250117193009.0 |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | fre |
| 042 ## - AUTHENTICATION CODE | |
| Authentication code | dc |
| 100 10 - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Chesnay, Adélaïde |
| Relator term | author |
| 245 00 - TITLE STATEMENT | |
| Title | Contribution of a molecular test for the diagnosis of genital infection with Trichomonas vaginalis and Mycoplasma genitalium |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2020.<br/> |
| 500 ## - GENERAL NOTE | |
| General note | 85 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | RésuméDans la présente étude, nous avons évalué un test moléculaire récemment commercialisé, le kit S-DiaMGTV ™ (Diagenode), qui permet la détection simultanée de Mycoplasma genitalium et Trichomonas vaginalis dans des échantillons urogénitaux. Les caractéristiques de performance du kit S-DiaMGTV ™ ont été comparées à celles d’une PCR maison pour la détection de M. genitalium et, pour la première fois, à la technique microscopique de l’état frais pour T. vaginalis, une méthode de routine utilisée en laboratoire de biologie médicale. Pour M. genitalium, sur 66 échantillons, deux négatifs avec la PCR maison ont été trouvés positifs avec le kit S-DiaMGTV ™ et deux positifs avec la PCR maison ont été trouvés négatifs avec le kit. Pour T. vaginalis, quatre échantillons ont été trouvés positifs par le test moléculaire. Parmi eux, deux ont été testés par l’observation de montage humide et un seul était positif. Le kit permet une augmentation de la détection de T. vaginalis même dans un pays à faible incidence, comme la France. Les performances du kit sont en faveur de son utilisation en pratique courante au laboratoire. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | In the present study, we assessed a recently-marketed molecular test, the S-DiaMGTV™ kit (Diagenode), which provides simultaneous detection of Mycoplasma genitalium and Trichomonas vaginalis in urogenital samples. Performance characteristics of the S-DiaMGTV™ kit were compared to an in-house PCR for detection of M. genitalium and, for first time, with direct observation of genital secretions in wet mounting microscopy for T. vaginalis, a routine laboratory method. For M. genitalium, out of 66 samples, two negative with the in-house PCR were found positive with the S-DiaMGTV™ kit and two positive with the in-house PCR were found negative with the kit. For T. vaginalis, four samples were found positive by the molecular test. Among them, two were previously tested by the wet mounting observation and only one was positive. The kit allows an increase of T. vaginalis detection even in a low incidence country. Performances of the kit are in favor of its use in routine laboratory practice. |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | sexually-transmitted infection (STI) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | real time PCR |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | trichomoniasis |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | trichomonose |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Trichomonas vaginalis |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | PCR en temps réel |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | infection sexuellement transmissible (IST) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Mycoplasma genitalium |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lancelin, Benoit |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Le Brun, Cécile |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Pastuszka, Adeline |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Desoubeaux, Guillaume |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lanotte, Philippe |
| Relator term | author |
| 786 0# - DATA SOURCE ENTRY | |
| Note | Annales de Biologie Clinique | 78 | 6 | 2020-11-01 | p. 623-627 | 0003-3898 |
| 856 41 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://shs.cairn.info/revue-annales-de-biologie-clinique-2020-6-page-623?lang=en&redirect-ssocas=7080">https://shs.cairn.info/revue-annales-de-biologie-clinique-2020-6-page-623?lang=en&redirect-ssocas=7080</a> |
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