In vitro activity of amphotericin B in combination with imipenem/colistin against mixed cultures of Candida spp. and Gram-negative bacteria (notice n° 276925)

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Personal name Halimi, Amel
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Title In vitro activity of amphotericin B in combination with imipenem/colistin against mixed cultures of Candida spp. and Gram-negative bacteria
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Date of publication, distribution, etc. 2024.<br/>
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General note 99
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Summary, etc. Background/purposeA new direction of research was established due to biofilms’ infections caused by a mixture of fungal and bacterial species. Diagnosis of these infections becomes more difficult and high doses of drugs are used in treatment, especially in critically ill patients. The aim of the current study was to examine the effects of amphotericin B, in combination with imipenem or colistin against Candida albicans - Acinetobacter baumannii- Proteus mirabilis and Candida tropicalis - Acinetobacter baumannii -Proteus mirabilis polymicrobial biofilms.Material and methodsAccording to the microdilution method of the Clinical Laboratory Standards Institute (CLSI) published in documents M27-A3 (2008) and M07-A10 (2015) respectively, the susceptibility of the clinical isolates Candida yeasts to amphotericin B and Gram-negative bacteria to imipenem and colistin was determined. A Checkerboard assay was employed to evaluate the efficacy of drugs combinations. The 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT) reduction assay was used to quantify the biofilms.ResultsThe combination amphotericin B/colistin and amphotericin B/imipenem did not produce obvious effects against the polymicrobial biofilms formed for 48 hours. Whereas colistin alone produced strong effects against Candida albicans-Acinetobacter baumannii and Candida albicans - Acinetobacter baumannii -Proteus mirabilis compared to Candida tropicalis - Acinetobacter baumannii and Candida tropicalis - Acinetobacter baumannii - Proteus mirabilis polymicrobial biofilms.ConclusionFor mixed Candida spp. bacterial biofilms, these results point to the difficulties in eradicating these biofilms, as well as in preventing their development.
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Summary, etc. Contexte/objectifUne nouvelle orientation de la recherche a été établie en raison des infections par biofilms causées par un mélange d’espèces fongiques et bactériennes. Le diagnostic de ces infections devient plus difficile et des doses élevées de médicaments sont utilisées dans le traitement, en particulier chez les patients gravement malades. L’objectif de la présente étude était d’examiner les effets de l’amphotéricine B, en combinaison avec l’imipenem ou la colistine contre les biofilms polymicrobiens Candida albicans - Acinetobacter baumannii- Proteus mirabilis et Candida tropicalis - Acinetobacter baumannii -Proteus mirabilis.Matériel et méthodesSelon la méthode de microdilution du Clinical Laboratory Standards Institute (CLSI) publiée dans les documents M27-A3 (2008) et M07-A10 (2015) respectivement, la sensibilité des isolats cliniques de levures Candida à l’amphotéricine B et des bactéries Gram négatif à l’imipénem et à la colistine a été déterminée. Un test Checkerboard a été utilisé pour évaluer l’efficacité des combinaisons de médicaments. Le test de réduction au bromure de3-(4,5-diméthylthiazol-2-yl) -2,5-diphényl-tétrazolium (MTT) a été utilisé pour quantifier les biofilms.RésultatsLa combinaison amphotéricine B/colistine et amphotéricine B/imipénème n’a pas produit d’effets évidents contre les biofilms polymicrobiens formés pendant 48 heures. En revanche, la colistine seule a produit des effets importants contre les biofilms polymicrobiens Candida albicans-Acinetobacter baumannii et Candida albicans - Acinetobacter baumannii -Proteus mirabilis par rapport aux biofilms polymicrobiens Candida tropicalis - Acinetobacter baumannii et Candida tropicalis - Acinetobacter baumannii - Proteus mirabilis.ConclusionPour les biofilms bactériens mixtes Candida spp., ces résultats soulignent les difficultés à éradiquer ces biofilms, ainsi qu’à prévenir leur développement.
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element combinaisons antimicrobiennes
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element biofilm polymicrobien
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element imipénème
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element combinaisons antimicrobiennes
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element biofilm polymicrobien
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element imipénème
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element combinaisons antimicrobiennes
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element biofilm polymicrobien
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element imipénème
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element combinaisons antimicrobiennes
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element biofilm polymicrobien
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element imipénème
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element combinaisons antimicrobiennes
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element biofilm polymicrobien
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element imipénème
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element combinaisons antimicrobiennes
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element biofilm polymicrobien
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element imipénème
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element combinaisons antimicrobiennes
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element biofilm polymicrobien
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element imipénème
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element biofilm polymicrobien
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element imipénème
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element combinaisons antimicrobiennes
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Topical term or geographic name as entry element biofilm polymicrobien
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element combinaisons antimicrobiennes
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Topical term or geographic name as entry element imipénème
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element biofilm polymicrobien
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Topical term or geographic name as entry element colistine
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Topical term or geographic name as entry element bactéries à Gram négatif
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Topical term or geographic name as entry element combinaisons antimicrobiennes
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Topical term or geographic name as entry element imipénème
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Topical term or geographic name as entry element amphotéricine B
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Topical term or geographic name as entry element Candida spp.
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Topical term or geographic name as entry element polymicrobial biofilm
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Topical term or geographic name as entry element colistin
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Topical term or geographic name as entry element Candida
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Topical term or geographic name as entry element gram-negative bacteria
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Topical term or geographic name as entry element imipenem
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Topical term or geographic name as entry element spp.
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Topical term or geographic name as entry element amphotericin B
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Topical term or geographic name as entry element antimicrobial combinations
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Topical term or geographic name as entry element polymicrobial biofilm
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Topical term or geographic name as entry element colistin
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Topical term or geographic name as entry element Candida
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Topical term or geographic name as entry element gram-negative bacteria
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Topical term or geographic name as entry element imipenem
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Topical term or geographic name as entry element spp.
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Topical term or geographic name as entry element amphotericin B
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Topical term or geographic name as entry element antimicrobial combinations
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Topical term or geographic name as entry element polymicrobial biofilm
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Topical term or geographic name as entry element colistin
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Topical term or geographic name as entry element Candida
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Topical term or geographic name as entry element gram-negative bacteria
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Topical term or geographic name as entry element imipenem
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Topical term or geographic name as entry element spp.
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Topical term or geographic name as entry element amphotericin B
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Topical term or geographic name as entry element antimicrobial combinations
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Topical term or geographic name as entry element polymicrobial biofilm
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Topical term or geographic name as entry element colistin
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Topical term or geographic name as entry element Candida
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Topical term or geographic name as entry element gram-negative bacteria
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Topical term or geographic name as entry element imipenem
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Topical term or geographic name as entry element spp.
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element amphotericin B
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Topical term or geographic name as entry element antimicrobial combinations
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Topical term or geographic name as entry element polymicrobial biofilm
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Topical term or geographic name as entry element colistin
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Topical term or geographic name as entry element Candida
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Topical term or geographic name as entry element gram-negative bacteria
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Topical term or geographic name as entry element imipenem
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Topical term or geographic name as entry element spp.
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Topical term or geographic name as entry element amphotericin B
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Topical term or geographic name as entry element antimicrobial combinations
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Topical term or geographic name as entry element polymicrobial biofilm
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Topical term or geographic name as entry element colistin
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Topical term or geographic name as entry element Candida
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Topical term or geographic name as entry element gram-negative bacteria
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Topical term or geographic name as entry element imipenem
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Topical term or geographic name as entry element spp.
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Topical term or geographic name as entry element amphotericin B
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Topical term or geographic name as entry element antimicrobial combinations
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Topical term or geographic name as entry element polymicrobial biofilm
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Topical term or geographic name as entry element colistin
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Topical term or geographic name as entry element Candida
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Topical term or geographic name as entry element gram-negative bacteria
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Topical term or geographic name as entry element imipenem
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Topical term or geographic name as entry element spp.
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Topical term or geographic name as entry element amphotericin B
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Topical term or geographic name as entry element antimicrobial combinations
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Topical term or geographic name as entry element polymicrobial biofilm
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Topical term or geographic name as entry element colistin
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Topical term or geographic name as entry element Candida
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Topical term or geographic name as entry element gram-negative bacteria
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element imipenem
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Topical term or geographic name as entry element spp.
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Topical term or geographic name as entry element amphotericin B
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Topical term or geographic name as entry element antimicrobial combinations
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Topical term or geographic name as entry element polymicrobial biofilm
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element colistin
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element Candida
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element gram-negative bacteria
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element imipenem
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element spp.
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element amphotericin B
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element antimicrobial combinations
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element Candida
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element antimicrobial combinations
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Topical term or geographic name as entry element polymicrobial biofilm
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Topical term or geographic name as entry element colistin
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element imipenem
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element amphotericin B
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element gram-negative bacteria
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element spp.
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element colistin
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element imipenem
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Topical term or geographic name as entry element spp.
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element polymicrobial biofilm
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Topical term or geographic name as entry element gram-negative bacteria
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element amphotericin B
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element Candida
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element antimicrobial combinations
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element colistin
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element imipenem
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element spp.
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element polymicrobial biofilm
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element gram-negative bacteria
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element amphotericin B
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element Candida
690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN)
Topical term or geographic name as entry element antimicrobial combinations
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Ahmed-KaziTani, Zahira Zakia Baba
Relator term author
700 10 - ADDED ENTRY--PERSONAL NAME
Personal name Boucherit-Otmani, Zahia
Relator term author
786 0# - DATA SOURCE ENTRY
Note Annales de Biologie Clinique | 82 | 5 | 2024-12-04 | p. 527-535 | 0003-3898
856 41 - ELECTRONIC LOCATION AND ACCESS
Uniform Resource Identifier <a href="https://shs.cairn.info/revue-annales-de-biologie-clinique-2024-5-page-527?lang=en&redirect-ssocas=7080">https://shs.cairn.info/revue-annales-de-biologie-clinique-2024-5-page-527?lang=en&redirect-ssocas=7080</a>

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