LncRNA RP11-773H22.4 is upregulated in severe pneumonia and may be a diagnostic and prognostic marker for severe pneumonia (notice n° 283853)
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| fixed length control field | 04453cam a2200301 4500500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250117203137.0 |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | fre |
| 042 ## - AUTHENTICATION CODE | |
| Authentication code | dc |
| 100 10 - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Cao, Yan |
| Relator term | author |
| 245 00 - TITLE STATEMENT | |
| Title | LncRNA RP11-773H22.4 is upregulated in severe pneumonia and may be a diagnostic and prognostic marker for severe pneumonia |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2024.<br/> |
| 500 ## - GENERAL NOTE | |
| General note | 63 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | BackgroundThe incidence of pneumonia has become increasingly prevalent, and its severity has been continuously escalating, bringing significant damage and stress to people’s lives. The regulatory role of RP11-773H22.4 in the onset and development of severe pneumonia is emerging as an important factor, however, the exact mechanisms controlling its effects have not been fully elucidated.MethodsROC curve and Kaplan-Meier curve were employed to assess the diagnostic and prognostic significance of RP11-773H22.4 in severe pneumonia. qRT-PCR was employed to assess the RP11-773H22.4 and miR-1287-5p expression. The CCK-8 was employed to assess cell viability. The rate of apoptosis was measured utilizing flow cytometric. The concentration of inflammatory factors was detected by ELISA kit. The interaction between RP11-773H22.4 and miR-1287-5p was verified by dual luciferase reporter gene assay.ResultsIn individuals afflicted with severe pneumonia, there was an observed up-regulation in RP11-773H22.4 expression and a corresponding decline in miR-1287-5p expression. RP11-773H22.4 demonstrated diagnostic and prognostic significance for severe pneumonia. RP11-773H22.4 augmented the viability of MRC-5 cells with LPS treatment by modulating miR-1287-5p, leading to a reduction in apoptosis and lower levels of inflammatory cytokines.ConclusionRP11-773H22.4 was highly expressed in severe pneumonia and may serve as a diagnostic and prognostic marker for severe pneumonia. miR-1287-5p was downregulated in severe pneumonia, and RP11-773H22.4 participated in the pathogenesis of severe pneumonia by regulating the expression of miR-1287-5p. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | ContexteL’incidence de la pneumonie est de plus en plus importante et sa gravité ne cesse d’augmenter, entraînant des dommages et un stress considérable dans la vie des patients. Le rôle régulateur de RP11-773H22.4 dans l’apparition et le développement de la pneumonie sévère apparaît comme un facteur important, mais les mécanismes exacts qui contrôlent ses effets n’ont pas encore été entièrement élucidés.MéthodesLa courbe ROC et la courbe de Kaplan-Meier ont été utilisées pour évaluer l’importance diagnostique et pronostique de RP11-773H22.4 dans la pneumonie sévère. La qRT-PCR a été utilisée pour évaluer l’expression de RP11-773H22.4 et de miR-1287-5p. Le CCK-8 a été utilisé pour évaluer la viabilité cellulaire. Le taux d’apoptose a été mesuré par cytométrie de flux. La concentration des facteurs inflammatoires a été détectée à l’aide d’un kit ELISA. L’interaction entre RP11-773H22.4 et miR-1287-5p a été vérifiée par un test de gène rapporteur à double luciférase.RésultatsChez les individus atteints de pneumonie sévère, on a observé une augmentation de l’expression de RP11-773H22.4 et une diminution correspondante de l’expression de miR-1287-5p. Le RP11-773H22.4 a démontré une importance diagnostique et pronostique pour la pneumonie sévère. RP11-773H22.4 a augmenté la viabilité des cellules MRC-5 traitées au LPS en modulant miR-1287-5p, ce qui a entraîné une réduction de l’apoptose et des niveaux inférieurs de cytokines inflammatoires.ConclusionRP11-773H22.4 était fortement exprimé dans la pneumonie sévère et peut servir de marqueur diagnostique et pronostique pour la pneumonie sévère. miR-1287-5p était régulé à la baisse dans la pneumonie sévère, et RP11-773H22.4 a participé à la pathogenèse de la pneumonie sévère en régulant l’expression de miR-1287-5p. |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | miR-1287-5p |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | diagnostic |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | RP11-773H22.4 |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | pneumonie grave |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | pronostic |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | miR-1287-5p |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | prognosis |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | RP11-773H22.4 |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | diagnosis |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | severe pneumonia |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Wang, Feiyan |
| Relator term | author |
| 786 0# - DATA SOURCE ENTRY | |
| Note | Annales de Biologie Clinique | 82 | 2 | 2024-03-01 | p. 187-199 | 0003-3898 |
| 856 41 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://shs.cairn.info/revue-annales-de-biologie-clinique-2024-2-page-187?lang=en&redirect-ssocas=7080">https://shs.cairn.info/revue-annales-de-biologie-clinique-2024-2-page-187?lang=en&redirect-ssocas=7080</a> |
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