Détection des auto-anticorps antithyroïdiens dans le LCR par technique d’immunofluorométrie (FEIA) : application au diagnostic biologique de l’encéphalopathie d’Hashimoto (notice n° 420502)
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| fixed length control field | 04466cam a2200421 4500500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250120125804.0 |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | fre |
| 042 ## - AUTHENTICATION CODE | |
| Authentication code | dc |
| 100 10 - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Serrier, Julien |
| Relator term | author |
| 245 00 - TITLE STATEMENT | |
| Title | Détection des auto-anticorps antithyroïdiens dans le LCR par technique d’immunofluorométrie (FEIA) : application au diagnostic biologique de l’encéphalopathie d’Hashimoto |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2021.<br/> |
| 500 ## - GENERAL NOTE | |
| General note | 21 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | RésuméL’encéphalopathie d’Hashimoto (EH) est une pathologie rare sous diagnostiquée s’exprimant par une hétérogénéité de symptômes neurologiques. Elle est associée à la présence de taux élevés d’anticorps antithyropéroxydase (TPO) et/ou antithyroglobuline (TG) dans le sang et le liquide céphalo-rachidien (LCR). Peu de techniques sont validées pour doser ces anticorps dans le LCR. Notre objectif a été d’adapter au LCR la technique de dosage sérique FEIA (Fluorescent-Enzyme Immuno Assay) de l’analyseur PhadiaTM 250 (EliATM, ThermoFisher Scientific) et d’évaluer ses performances. Les seuils de détection et de quantification pour chaque auto-anticorps ont été déterminés. Nous avons comparé cette technique à notre technique ELISA (Blanchin S. 2007) (concordance de 75 % et de 100 % pour les anti-TPO et les anti-TG respectivement), calculé les coefficients de variation (CV) intra- et inter-essais et l’incertitude de mesure. Notre étude montre que la technique FEIA adaptée au LCR, a une bonne corrélation avec la technique ELISA et de bonnes performances analytiques. La disponibilité de cet analyseur dans de nombreux laboratoires la rend facilement applicable au diagnostic de l’EH. |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | Hashimoto encephalopathy (HE) is a rare condition often underdiagnosed. The clinical picture is heterogeneous with numerous neurological signs and is associated with the presence of high levels of anti-thyroperoxidase (TPO) and / or anti-thyroglobulin (TG) antibodies in the blood and cerebrospinal fluid (CSF). The determination of anti-TPO and anti-TG antibodies in CSF is performed in only few laboratories. The aim of our study was to adapt the EliATM fluoroenzymatic immuno assay (FEIA) to the detection of these autoantibodies in the CSF, and to compare the results with our previously published ELISA test (Blanchin S. 2007). For the FEIA technique, the detection threshold, and the quantification threshold have been determined for anti-TPO and anti-TG antibodies. FEIA results were concordant with ELISA at 75% and 100% for anti-TPO and anti-TG antibodies, respectively. Coefficients of variation (CV) of the intra-assay and inter-assay results were calculated as well as the uncertainties of measurement. The anti-TPO and anti-TG antibodies detection in CSF using FEIA technique correlate with the previously published ELISA and show good analytical performances. The availability of PhadiaTM 250 analyzer in a large number of laboratories will allow an easier biological detection. We hope that this test will respond to physician needs and help for HE diagnosis. |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | encéphalopathie d’Hashimoto (EH) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Steroid-Responsive Encephalopathy Associated with auto-immune Thyroiditis (SREAT) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | anticorps anti-thyroperoxydase (anti-TPO) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | anticorps anti-thyroglobuline (anti-TG) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Liquide céphalo-rachidien (LCR) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Encephalopathy Associated with Auto-immune Thyroid disease (EAAT) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Fluorescent-Enzyme Immuno Assay (FEIA) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Steroid-Responsive Encephalopathy Associated with auto-immune Thyroiditis (SREAT) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Hashimoto's Encephalopathy (HE) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Anti-thyroglobulin antibodies (anti-TG) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Encephalopathy Associated with Auto-immune Thyroid disease (EAAT) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Fluorescent-Enzyme Immuno Assay (FEIA) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Anti-thyroperoxydase antibodies (anti-TPO) |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | Cerebrospinal fluid (CSF) |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Branger, Pierre |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Gondolf, Clémentine |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Khoy, Kathy |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Derache, Nathalie |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Le Mauff, Brigitte |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Comby, Elisabeth |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Mariotte, Delphine |
| Relator term | author |
| 786 0# - DATA SOURCE ENTRY | |
| Note | Annales de Biologie Clinique | 79 | 2 | 2021-03-01 | p. 159-167 | 0003-3898 |
| 856 41 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://shs.cairn.info/revue-annales-de-biologie-clinique-2021-2-page-159?lang=fr&redirect-ssocas=7080">https://shs.cairn.info/revue-annales-de-biologie-clinique-2021-2-page-159?lang=fr&redirect-ssocas=7080</a> |
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