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The ecotourism sector has the highest rate of growth in the world. It includes various activities such as hiking, cycling, climbing, and other survival activities (Santarém, Silva, and Santos, 2015). Ecological tourism is the subject of various projects and visions in Tunisia, with a view to improving the attractiveness of the country and preserving the resources and well-being of Tunisians. The purpose of this study is to understand hiking tourists’ behavior by exploring their motivations, quality of life, subjective well-being, and behavioral intentions after a hiking trip. A mixed-method of qualitative and quantitative analysis was applied to test the theoretical and empirical evidence of the relationships between these four parameters and to contribute to the development and promotion of this activity in Tunisia.