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Objectives: The aim of this study was to evaluate the well-being and occupational perception of healthcare workers in Morocco. Methods: This observational and cross-sectional survey, carried out by self-administered questionnaire, was conducted in public hospitals in three cities. The target population consisted of 2,453 healthcare workers, comprising physicians and paramedics with at least two years of clinical experience. Results: 1951 healthcare workers returned the questionnaire (29.4% physicians and 70.6% allied health personnel).The mean age was 40.3 ± 10.1 years. 79.8% drank tea and 59.6% drank coffee daily to remain alert at work. 13.9% were smokers and 5.5% drank alcohol. Medication use consisted of analgesics for 28.1% and psychotropic drugs for 11.6%. The respective prevalence of general health perception was “bad or very bad” for 14.1% to 24.5%. Perceived symptoms varied from 20.4% to 26.2%. Pain and headache were the most common symptoms. 53.9% “often and continuously” experienced stress at work. Work requirements were considered to be “difficult” or “very difficult” by 32.1% to 46.2%.The workplace was unsuitable for 37.2%, salary was inadequate for 39.4%, the workload was excessive for 39.8% and the work rate was excessive for 43.2%. Relationship with colleagues (work environments and trust) were appropriate for 55.6% and 65%, respectively. Relationships with hierarchical superiors were appropriate for 15.6% and based on confidence for 13.8%. Perceived social support outside of work was high. Conclusion: Assessment of well-being and occupational risk perception among healthcare workers must be one of the priority tasks of occupational health utilities in Moroccan hospitals.