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Introduction: Alternate work is known to cause physical and mental health disorders for employees that can alter their quality of life and sleep. Objective: The purpose of this research was to evaluate workers quality of life and sleep in situations of alternate work. Results: In total, 391 workers answered the questionnaire. According to the type of schedule, 320 employees worked alternately on a 2×8 shift schedule and 71 worked on a permanent night shift schedule. The group of employees working at night had a better quality of life than the group of employees working in 2×8 in terms of both their physical and mental scores (P < 10–3). Type 2×8 workers were found to have excessive daytime sleepiness as compared to those on fixed night schedules. Conclusions: Shortly, we can deduce from this study that alternating day shift work causes a deterioration of workers’ quality of life of and generates sleep disorders.