TY  - BOOK
AU  - Farfour,Eric
AU  -  Pascreau,Tiffany
AU  -  Zia-Chahabi,Sara
AU  -  Jolly,Emilie
AU  -  Ferrière,Patrice
AU  -  Mazaux,Laurence
AU  -  Vasse,Marc
TI  - Comparison of two SARS-CoV-2 RT-PCR assays and the implication of the instrument software on the cycle threshold (Ct) value
PY  - 2022///.
							
N1  - 47
N2  - The Cycle Threshold (Ct) value of SARS-CoV-2 RT-PCRs is used as an indicator of viral load. Using a collection of 45 fresh nasopharyngeal samples collected on universal transport media, we compare the Ct value obtained with 2 RT-PCR assays, the Alinity M SARS-CoV-2, and the Alinity M RESP-4-Plex (Abbott Molecular, Des Plaines, Illinois, United States) processed on an Alinity M device. The assays are highly correlated; however, the Ct values were a median of 4.54 lower with the Alinity M RESP-4-Plex. This difference could be attributed to earlier detection of positivity by the Alinity M software rather than a difference in RT-PCR performances. The Ct-value of SARS-CoV-2 RT-PCRs should be interpreted with caution, taking into account the clinical context and pre-analytical and analytical findings
UR  - https://shs.cairn.info/journal-annales-de-biologie-clinique-2022-6-page-537?lang=en
ER  -