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Introduction: Sex education should develop biological and psychosocial skills, but organizing such an approach remains challenging. The multidisciplinary primary care team of Montval-sur-Loir (Sarthe) developed a school-based psychosocial and sexual prevention program (PSPP), targeting 8th grade pupils in the two local middle-schools in Montval-sur-Loir. The aim of this study was to evaluate the impact of the PSPP on pupils’ knowledge and psychosocial representations about sexual health. Purpose of research: Eighth grade students from two different schools in Montval-sur-Loir and two different schools in Craon filled in a survey at the beginning and at the end of the school year. The PSPP took place in Montval-sur-Loir (intervention group) but not in Craon (control group). From the surveys, 12 questions were selected to create a sexual health knowledge score. The change in scores between intervention and control groups were compared using a multi-level regression. Secondary analysis examined every survey response individually. Results: Pupils who took part in the PSPP improved their score by an average of 1.7 points out of 12, which differs significantly from the control group. Additionally, two questions assessing social representations showed a significantly different evolution between intervention and control group. Conclusions: PSPP positively influenced pupils’ knowledge about sexual health, while its impact on psychosocial representations remains to be demonstrated. Broader and longer-term evaluation may be needed.