000			01821cam a2200217 4500500
005			20250112063653.0
041			_afre
042			_adc
100	1	0	_aMayran, Charly _eauthor
700	1	0	_a Henry, Steven _eauthor
700	1	0	_a Pinchon, Elena _eauthor
700	1	0	_a Fournier-Wirth, Chantal _eauthor
700	1	0	_a Cantaloube, Jean François _eauthor
700	1	0	_a Foulongne, Vincent _eauthor
245	0	0	_aCRISPR-Cas: The bacterial immunity that supports diagnostics in virology
260			_c2022.
500			_a34
520			_aCRISPR-Cas is an adaptive immune system that enables bacteria and archaea from nucleic acid to fight invasions such as viral genomes. The ability of the CRISPR-Cas technology to effectively and precisely cleave a targeted genomic DNA region has been utilized to develop powerful genome editing tools that have been adapted for a wide range of applications, revolutionizing biological sciences. The CRISPR-Cas system consists of a Cas endonuclease triggered by an RNA guide for highly specific cleavage of targeted DNA or RNA sequences. In addition to the target-specific cleavage, some Cas enzymes, including Cas12a and Cas13a, display a collateral trans-cleavage activity that allows the cleavage of all surrounding single-stranded nucleic acids. These biosensing activities of CRISPR-Cas systems, based on target-specific binding and cleavage, are promising tools for developing accurate diagnostic methods to detect specific nucleic acids. CRISPR-Cas could therefore be used to diagnose a wide variety of diseases. In this review, we describe the more significant advances for virus detection based on CRISPR-Cas systems.
786	0		_nVirologie | 26 | 4 | 2022-07-01 | p. 303-313 | 1267-8694
856	4	1	_uhttps://shs.cairn.info/journal-virologie-2022-4-page-303?lang=en
999			_c234792 _d234792