| 000 | 02610cam a2200301 4500500 | ||
|---|---|---|---|
| 005 | 20250112063928.0 | ||
| 041 | _afre | ||
| 042 | _adc | ||
| 100 | 1 | 0 |
_aBagheri, Yasser _eauthor |
| 700 | 1 | 0 |
_a Babaha, Fateme _eauthor |
| 700 | 1 | 0 |
_a Falak, Reza _eauthor |
| 700 | 1 | 0 |
_a Yazdani, Reza _eauthor |
| 700 | 1 | 0 |
_a Azizi, Gholamreza _eauthor |
| 700 | 1 | 0 |
_a Sadri, Maryam _eauthor |
| 700 | 1 | 0 |
_a Abolhassani, Hassan _eauthor |
| 700 | 1 | 0 |
_a Shekarabi, Mehdi _eauthor |
| 700 | 1 | 0 |
_a Aghamohammadi, Asghar _eauthor |
| 245 | 0 | 0 | _aIL-10 induces TGF-β secretion, TGF-β receptor II upregulation, and IgA secretion in B cells |
| 260 | _c2019. | ||
| 500 | _a43 | ||
| 520 | _aBackgroundInterleukin-10 (IL-10) is a pleiotropic cytokine, which has both regulatory and stimulatory effects on different immune cell types. Different studies have reported the importance of IL-10 and Transforming growth factor-beta (TGF-β) in the regulation of B cell class switching the production of immunoglobulin A (IgA); however, the underlying mechanisms remain to be fully elucidated. The objective of this study was to investigate the TGF-β response during B stimulation of human B cells by IL-10. MethodsPan B cells of healthy donors were negatively purified by a magnetic cell separation technique. B cells were cultured with multimeric CD40 ligand (mCD40L) and IL-10 for two and seven days. After harvesting in specific days, TGF-β receptor II and surface IgA expression was determined by flow cytometry, while IgA and TGF-β secretion was assessed by enzyme-linked immunosorbent assay. ResultsB cells endogenously expressed TGF-β receptor II and after 48 hours cultivation with mCD40L or mCD40L plus IL-10, both the expression of this receptor and the production of TGF-β were significantly increased. Notably, TGF-β levels following stimulation with mCD40L and IL-10 were higher than those produced by B cells stimulated with mCD40L alone. Furthermore, at day 7 and following IL-10 stimulation, there was a significant rise in the amount of IgA secretion by class-switched plasma cells, which was higher than stimulation with mCD40L alone. ConclusionOur findings suggest that IL-10 can modulate TGF-β production and TGF-β receptor expression in mCD40-activated human B lymphocytes. | ||
| 690 | _aimmunoglobulin A | ||
| 690 | _ainterleukin-10 | ||
| 690 | _atransforming growth factor-β | ||
| 690 | _aclass switching | ||
| 786 | 0 | _nEuropean Cytokine Network | Volume 30 | 3 | 2019-09-01 | p. 107-113 | 1148-5493 | |
| 856 | 4 | 1 | _uhttps://shs.cairn.info/revue-european-cytokine-network-2019-3-page-107?lang=en |
| 999 |
_c235854 _d235854 |
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