000			02161cam a2200265 4500500
005			20250112064120.0
041			_afre
042			_adc
100	1	0	_aBayati, Fatemeh _eauthor
700	1	0	_a Valadi, Maryam _eauthor
700	1	0	_a Ahmadi, Armin _eauthor
700	1	0	_a Najafi, Farangis _eauthor
700	1	0	_a Ansaripour, Bita _eauthor
700	1	0	_a Sharif-Paghaleh, Ehsan _eauthor
245	0	0	_aEvaluation of immunomodulatory effects of co-culture or supernatant of dexamethasone or IFN-γ-treated adipose-derived mesenchymal stem cells on spleen mononuclear cells
260			_c2022.
500			_a67
520			_aAlthough mesenchymal stem cells (MSCs) have exhibited promising immunomodulatory potential in preclinical studies, clinical studies have revealed variable results. These results often depend on environmental cues. Pre-conditioning MSCs with cytokines is one of the methods used to enhance their immunomodulatory effects. In this study, we harvested adipose-derived MSCs from mice and cultured them with different doses of the cytokine, IFN-γ, and the corticosteroid drug, dexamethasone, in order to investigate their effects on MSC immunosuppressive function. We found the co-culture or supernatant of MSCs, pre-conditioned with IFN-γ, together with spleen mononuclear cells resulted in a significant reduction of mononuclear cell proliferation. Although the supernatant of MSCs, pre-conditioned with dexamethasone, showed similar results, dexamethasone pre-conditioning of co-cultured MSCs increased mononuclear cell proliferation. The results further our understanding of immune-related effects of MSCs which may provide a basis for further in vivo studies to achieve better clinical results. We propose that pre-conditioning with cytokines might be an effective method to boost the immunomodulatory effects of MSCs.
690			_amesenchymal stem cells
690			_aimmunomodulation
690			_aIFN-γ
690			_adexamethasone
786	0		_nEuropean Cytokine Network | Volume 33 | 3 | 2022-09-01 | p. 70-78 | 1148-5493
856	4	1	_uhttps://shs.cairn.info/revue-european-cytokine-network-2022-3-page-70?lang=en
999			_c236488 _d236488