| 000 | 04466cam a2200421 4500500 | ||
|---|---|---|---|
| 005 | 20250120170530.0 | ||
| 041 | _afre | ||
| 042 | _adc | ||
| 100 | 1 | 0 |
_aSerrier, Julien _eauthor |
| 700 | 1 | 0 |
_a Branger, Pierre _eauthor |
| 700 | 1 | 0 |
_a Gondolf, Clémentine _eauthor |
| 700 | 1 | 0 |
_a Khoy, Kathy _eauthor |
| 700 | 1 | 0 |
_a Derache, Nathalie _eauthor |
| 700 | 1 | 0 |
_a Le Mauff, Brigitte _eauthor |
| 700 | 1 | 0 |
_a Comby, Elisabeth _eauthor |
| 700 | 1 | 0 |
_a Mariotte, Delphine _eauthor |
| 245 | 0 | 0 | _aDétection des auto-anticorps antithyroïdiens dans le LCR par technique d’immunofluorométrie (FEIA) : application au diagnostic biologique de l’encéphalopathie d’Hashimoto |
| 260 | _c2021. | ||
| 500 | _a21 | ||
| 520 | _aRésuméL’encéphalopathie d’Hashimoto (EH) est une pathologie rare sous diagnostiquée s’exprimant par une hétérogénéité de symptômes neurologiques. Elle est associée à la présence de taux élevés d’anticorps antithyropéroxydase (TPO) et/ou antithyroglobuline (TG) dans le sang et le liquide céphalo-rachidien (LCR). Peu de techniques sont validées pour doser ces anticorps dans le LCR. Notre objectif a été d’adapter au LCR la technique de dosage sérique FEIA (Fluorescent-Enzyme Immuno Assay) de l’analyseur PhadiaTM 250 (EliATM, ThermoFisher Scientific) et d’évaluer ses performances. Les seuils de détection et de quantification pour chaque auto-anticorps ont été déterminés. Nous avons comparé cette technique à notre technique ELISA (Blanchin S. 2007) (concordance de 75 % et de 100 % pour les anti-TPO et les anti-TG respectivement), calculé les coefficients de variation (CV) intra- et inter-essais et l’incertitude de mesure. Notre étude montre que la technique FEIA adaptée au LCR, a une bonne corrélation avec la technique ELISA et de bonnes performances analytiques. La disponibilité de cet analyseur dans de nombreux laboratoires la rend facilement applicable au diagnostic de l’EH. | ||
| 520 | _aHashimoto encephalopathy (HE) is a rare condition often underdiagnosed. The clinical picture is heterogeneous with numerous neurological signs and is associated with the presence of high levels of anti-thyroperoxidase (TPO) and / or anti-thyroglobulin (TG) antibodies in the blood and cerebrospinal fluid (CSF). The determination of anti-TPO and anti-TG antibodies in CSF is performed in only few laboratories. The aim of our study was to adapt the EliATM fluoroenzymatic immuno assay (FEIA) to the detection of these autoantibodies in the CSF, and to compare the results with our previously published ELISA test (Blanchin S. 2007). For the FEIA technique, the detection threshold, and the quantification threshold have been determined for anti-TPO and anti-TG antibodies. FEIA results were concordant with ELISA at 75% and 100% for anti-TPO and anti-TG antibodies, respectively. Coefficients of variation (CV) of the intra-assay and inter-assay results were calculated as well as the uncertainties of measurement. The anti-TPO and anti-TG antibodies detection in CSF using FEIA technique correlate with the previously published ELISA and show good analytical performances. The availability of PhadiaTM 250 analyzer in a large number of laboratories will allow an easier biological detection. We hope that this test will respond to physician needs and help for HE diagnosis. | ||
| 690 | _aencéphalopathie d’Hashimoto (EH) | ||
| 690 | _aSteroid-Responsive Encephalopathy Associated with auto-immune Thyroiditis (SREAT) | ||
| 690 | _aanticorps anti-thyroperoxydase (anti-TPO) | ||
| 690 | _aanticorps anti-thyroglobuline (anti-TG) | ||
| 690 | _aLiquide céphalo-rachidien (LCR) | ||
| 690 | _aEncephalopathy Associated with Auto-immune Thyroid disease (EAAT) | ||
| 690 | _aFluorescent-Enzyme Immuno Assay (FEIA) | ||
| 690 | _aSteroid-Responsive Encephalopathy Associated with auto-immune Thyroiditis (SREAT) | ||
| 690 | _aHashimoto's Encephalopathy (HE) | ||
| 690 | _aAnti-thyroglobulin antibodies (anti-TG) | ||
| 690 | _aEncephalopathy Associated with Auto-immune Thyroid disease (EAAT) | ||
| 690 | _aFluorescent-Enzyme Immuno Assay (FEIA) | ||
| 690 | _aAnti-thyroperoxydase antibodies (anti-TPO) | ||
| 690 | _aCerebrospinal fluid (CSF) | ||
| 786 | 0 | _nAnnales de Biologie Clinique | 79 | 2 | 2021-03-01 | p. 159-167 | 0003-3898 | |
| 856 | 4 | 1 | _uhttps://shs.cairn.info/revue-annales-de-biologie-clinique-2021-2-page-159?lang=fr&redirect-ssocas=7080 |
| 999 |
_c420531 _d420531 |
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