000 02934cam a2200349 4500500
005 20250121154620.0
041 _afre
042 _adc
100 1 0 _aGiavina-Bianchi, Mara
_eauthor
700 1 0 _a Giavina-Bianchi, Pedro
_eauthor
700 1 0 _a Sotto, Mirian Nacagamo
_eauthor
700 1 0 _a Rodig, Scott
_eauthor
700 1 0 _a Mihm Jr, Martin
_eauthor
700 1 0 _a Festa Neto, Cyro
_eauthor
700 1 0 _a Duncan, Lyn M.
_eauthor
700 1 0 _a Kalil, Jorge
_eauthor
245 0 0 _aNodular primary cutaneous melanoma is associated with PD-L1 expression
260 _c2020.
500 _a75
520 _aBackground: In previous studies, patients with Stage III melanomas expressing PD-L1 in more than 5% of their neoplastic cells had improved recurrence-free survival with anti-PD1 adjuvant therapy. Objectives: We examined PD-L1 expression as a possible biomarker of primary cutaneous melanomas in the vertical growth phase. Materials and Methods: This was a retrospective study including 66 patients with invasive primary cutaneous melanomas. We assessed patient clinical and histopathological data and performed immunohistochemical assays with melanoma specimens from the patients to evaluate PD-L1, PD-1, CD3, CD8 and FoxP3 expression. Results: We observed PD-L1 expression in 21% (14/66) of our samples, and this expression correlated with increased melanoma thickness (p = 0.002) and nodular-type melanoma (p = 0.001). After adjusting for tumor thickness using a logistic regression test, the association of PD-L1 with nodular-type melanoma persisted. Nodular-type melanoma was 6.48 times more likely to be positive for PD-L1 than other histological types (p = 0.014; 95% CI: 1.46-28.82). As expected, PD-L1 expression correlated with the number of PD-1-expressing cells in the tumor-infiltrating lymphocyte population (p = 0.04). No correlation with PD-L1 was observed for age, sex, tumor site, skin phototype, ulceration status, sentinel lymph node status, metastasis development or survival. Regarding the immune profile of the tumor-infiltrating lymphocytes of PD-L1-positive and -negative groups, no significant differences were observed in the numbers of CD3 + , CD8 + FoxP3-, CD8-FoxP3+ and CD8 + FoxP3+ cells by immunohistochemistry. Conclusion: Nodular-type melanoma is associated with PD-L1 expression and may be a suitable candidate for adjuvant therapy of primary melanomas treated with immunotherapy.
690 _anodular melanoma
690 _atumor infiltrating lymphocytes
690 _aPD-1
690 _aPD-L1
690 _atumor microenvironment
690 _aCD8
690 _aBreslow index
690 _aFoxP3
690 _aCD3
786 0 _nEuropean Journal of Dermatology | 30 | 4 | 2020-07-01 | p. 352-357 | 1167-1122
856 4 1 _uhttps://shs.cairn.info/revue-european-journal-of-dermatology-2020-4-page-352?lang=en&redirect-ssocas=7080
999 _c602798
_d602798