| 000 | 02501cam a2200265 4500500 | ||
|---|---|---|---|
| 005 | 20250121155354.0 | ||
| 041 | _afre | ||
| 042 | _adc | ||
| 100 | 1 | 0 |
_aSeidel, Katharina _eauthor |
| 700 | 1 | 0 |
_a Lutz, Fanny _eauthor |
| 700 | 1 | 0 |
_a Braun, Christian _eauthor |
| 700 | 1 | 0 |
_a Mann, Miriam _eauthor |
| 700 | 1 | 0 |
_a Kerschnitzki, Anette _eauthor |
| 700 | 1 | 0 |
_a Tietze, Julia K. _eauthor |
| 245 | 0 | 0 | _aEvaluation of the heat sensitivity of Trichophyton rubrum and Trichophyton interdigitale |
| 260 | _c2023. | ||
| 500 | _a93 | ||
| 520 | _aBackground: Onychomycosis affects up to 50% of patients in the older population. Objectives: This study aimed to explore heat sensitivity of Trichophyton rubrum and Trichophyton interdigitale as pathogens of onychomycosis. Materials & Methods: The fungi were heated in sterile saline solution up to 100°C for five or 10 minutes with or without additional previous treatment with 1% ciclopirox solution or chitinase and 1,3 -galactidase or for 45 minutes at 40°C or 60°C with washing powder. Subsequently, the fungi were cultured and regrowth was assessed after one week. Results: After heating T. rubrum for five minutes at 60°C, growth was completely inhibited. After heating T. interdigitale for five minutes at 60°C, all of the samples regrew, and at 95°C, none of the samples regrew. No difference between five and 10-minute heating was observed. Previous incubation with 1% ciclopirox solution for 24 hours inhibited the growth of T. rubrum completely. T. interdigitale was still able to regrow to 100% after five minutes at 40°C, to 33% after 60°C, and to 22% after 80°C. Incubation for 45 minutes with washing powder solution at 40°C or 60°C did not lead to significant growth reduction of T. rubrum or interdigitale. Two hours incubation with -1,3-glucanase and chitinase prior to five minutes of heating to 60°C and 80°C reduced the heat resistance of T. interdigitale; growth was inhibited in 56% and 100% of the samples, respectively. Conclusion: The heat resistance of T. rubrum and interdigitale should be considered using non-medical thermal treatment. | ||
| 690 | _aheat resistance thermal treatment | ||
| 690 | _aonychomycosis | ||
| 690 | _aTrichophyton interdigitale | ||
| 690 | _aTrichophyton rubrum | ||
| 786 | 0 | _nEuropean Journal of Dermatology | 33 | 1 | 2023-01-01 | p. 19-24 | 1167-1122 | |
| 856 | 4 | 1 | _uhttps://shs.cairn.info/revue-european-journal-of-dermatology-2023-1-page-19?lang=en&redirect-ssocas=7080 |
| 999 |
_c604606 _d604606 |
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