| 000 | 01900cam a2200289 4500500 | ||
|---|---|---|---|
| 005 | 20250123095910.0 | ||
| 041 | _afre | ||
| 042 | _adc | ||
| 100 | 1 | 0 |
_aZocchi, M. _eauthor |
| 700 | 1 | 0 |
_a Scrimieri, R. _eauthor |
| 700 | 1 | 0 |
_a Locatelli, L. _eauthor |
| 700 | 1 | 0 |
_a Cazzaniga, Antonio _eauthor |
| 700 | 1 | 0 |
_a Fedele, G. _eauthor |
| 700 | 1 | 0 |
_a Maier, Jeanette A. _eauthor |
| 700 | 1 | 0 |
_a Castiglioni, Sara _eauthor |
| 245 | 0 | 0 | _aTRPM7 and MagT1 regulate the proliferation of osteoblast-like SaOS-2 cells through different mechanisms |
| 260 | _c2020. | ||
| 500 | _a30 | ||
| 520 | _aA correct magnesium (Mg2+) intake is essential for bone health. In particular, Mg2+ deficiency inhibits the proliferation of osteoblast-like SaOS-2 cells by increasing nitric oxide (NO) production through the upregulation of inducible NO synthase. At the moment, little is known about the expression and the role of TRPM7, a channel/enzyme involved in Mg2+ uptake, and MagT1, a Mg2+ selective transporter, in SaOS-2 cells. Here, we demonstrate that TRPM7 is not modulated by different extracellular concentrations of Mg2+ and its silencing exacerbates growth inhibition exerted by low Mg2+ through the activation of inducible NO synthase and consequent accumulation of NO. Moreover, MagT1 is upregulated in SaOS-2 cultured in high Mg2+ and its silencing inhibits the growth of SaOS-2 cultured in media containing physiological or high Mg2+, without any modulation of NO production. We propose that TRPM7 and MagT1 are both involved in regulating SaOS-2 proliferation through different mechanisms. | ||
| 690 | _aTRPM7 | ||
| 690 | _aosteoblasts | ||
| 690 | _aMagT1 | ||
| 690 | _aMagnesium | ||
| 690 | _abone | ||
| 786 | 0 | _nMagnesium Research | 33 | 1 | 2020-01-01 | p. 12-20 | 0953-1424 | |
| 856 | 4 | 1 | _uhttps://shs.cairn.info/revue-magnesium-research-2020-1-page-12?lang=en&redirect-ssocas=7080 |
| 999 |
_c755367 _d755367 |
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