Validation d’une méthode de mesure de l’activité antiélastasique de l’alpha1-antitrypsine circulante humaine (notice n° 276958)
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| fixed length control field | 03771cam a2200361 4500500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20250117194132.0 |
| 041 ## - LANGUAGE CODE | |
| Language code of text/sound track or separate title | fre |
| 042 ## - AUTHENTICATION CODE | |
| Authentication code | dc |
| 100 10 - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Dechomet, Magali |
| Relator term | author |
| 245 00 - TITLE STATEMENT | |
| Title | Validation d’une méthode de mesure de l’activité antiélastasique de l’alpha1-antitrypsine circulante humaine |
| 260 ## - PUBLICATION, DISTRIBUTION, ETC. | |
| Date of publication, distribution, etc. | 2024.<br/> |
| 500 ## - GENERAL NOTE | |
| General note | 76 |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | The existence of alpha-1 antitrypsin variants with apparently unremarkable phenotypes and serum concentrations, contrasting with a clinical picture suggestive of a severe deficiency, led us to investigate whether in these cases there was a reduction or even suppression of the capacity of alpha-1 antitrypsin to inhibit elastase. To this end, in two different laboratories, we adapted and validated a method for measuring the functional activity of alpha-1 antitrypsin, based on spectrophotometric kinetic analysis of the inhibition by serum alpha-1 antitrypsin of the hydrolytic activity of porcine pancreatic elastase on a chromogenic substrate.This method has proved to be robust, reproducible and transferable and made possible to define, on the basis of an analysis of a hospital population, a functionality index with a confidence interval comprised between 0.87 and 1.2, allowing to identify subjects likely to have a functional deficiency of alpha-1 antitrypsin, whether this deficiency being of a genetic origin without any quantitative or phenotypic translation, or whether being acquired under the effect of external agents (cigarette smoke or viruses). |
| 520 ## - SUMMARY, ETC. | |
| Summary, etc. | L’existence de variants d’alpha-1 antitrypsine à phénotype et concentration sérique apparemment sans particularités contrastant avec un tableau clinique évocateur de l’existence d’un déficit sévère nous a conduits à rechercher dans ces cas de figure une diminution voire une suppression de la capacité de l’alpha-1 antitrypsine à inhiber l’élastase. Pour ce faire et dans deux laboratoires différents, nous avons adapté et validé une méthode de mesure de l’activité fonctionnelle de l’alpha-1 antitrypsine dont le principe repose sur l’analyse spectrophotométrique cinétique de l’inhibition par l’alpha-1 antitrypsine sérique de l’activité hydrolytique de l’élastase pancréatique de porc sur un substrat chromogène.Cette méthode s’est avérée robuste, reproductible et transférable et a permis de définir à partir de l’analyse d’une population hospitalière, un index de fonctionnalité dont l’intervalle de confiance à 95 % compris entre 0,87 et 1,2 permet d’identifier les sujets susceptibles de présenter un déficit fonctionnel de l’alpha-1 antitrypsine, que ce déficit soit d’origine génétique mais sans traduction quantitative et phénotypique ou bien que ce déficit soit acquis sous l’effet d’agents extérieurs (fumée de cigarettes ou virus). |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | pouvoir antiélastasique |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | validation de méthode |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | déficit en alpha1-antitrypsine |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | pouvoir antiélastasique |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | validation de méthode |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | déficit en alpha1-antitrypsine |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | alpha1-antitrypsin deficiency |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | method validation |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | elastase-inhibitory capacity |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | alpha1-antitrypsin deficiency |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | method validation |
| 690 ## - LOCAL SUBJECT ADDED ENTRY--TOPICAL TERM (OCLC, RLIN) | |
| Topical term or geographic name as entry element | elastase-inhibitory capacity |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Zerimech, Farid |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Chapuis-Cellier, Colette |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Lombard, Christine |
| Relator term | author |
| 700 10 - ADDED ENTRY--PERSONAL NAME | |
| Personal name | Balduyck, Malika |
| Relator term | author |
| 786 0# - DATA SOURCE ENTRY | |
| Note | Annales de Biologie Clinique | 82 | 3 | 2024-07-03 | p. 308-320 | 0003-3898 |
| 856 41 - ELECTRONIC LOCATION AND ACCESS | |
| Uniform Resource Identifier | <a href="https://shs.cairn.info/revue-annales-de-biologie-clinique-2024-3-page-308?lang=fr&redirect-ssocas=7080">https://shs.cairn.info/revue-annales-de-biologie-clinique-2024-3-page-308?lang=fr&redirect-ssocas=7080</a> |
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